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pcdna4  (Addgene inc)


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    Addgene inc pcdna4
    Pcdna4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+snail+wt+plasmids/pm39532099-238-107-130?v=Addgene+inc
    Average 93 stars, based on 16 article reviews
    pcdna4 - by Bioz Stars, 2026-07
    93/100 stars

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    Fig. 3. PML interacts with EMT factors and negatively correlates with TWIST2 expression. (A) qRT-PCR of MDA-MB-231, control and PML- KD cells for <t>TWIST1</t> & TWIST2 expression. Results are mean SD (n = 3). t-Test **P-value ≤0.05. (B) Inverse correlation of PML–TWIST2 RNA expression levels (log2 microarray from the Metabric dataset). (C) Confocal images (representative of four experiments) of co- localization between PML isoform IV, fused to mRED, and TWIST2 (upper panels) or SNAI2 (lower panels) fused to GFP (scale bar 5 lm). (D) Western blotting (WB) of co-immunoprecipitation of endogenous PML and TWIST2 proteins in MDA-MB 231 cells. Input (IN) or immuno- precipitated (IP) proteins were detected by an anti-PML (IP : PML) or IgG (IP : IgG) used as negative control (n = 2). (E) Co- immunoprecipitation of PML with TWIST1, TWIST2, SNAIL1, and SLUG (SNAI2) following co-expression in HEK293T cells. Input (IN) or anti- PML antibody immunoprecipitated (IP) proteins were detected by anti-GFP (WB : GFP; n = 2).
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    Image Search Results


    Fig. 3. PML interacts with EMT factors and negatively correlates with TWIST2 expression. (A) qRT-PCR of MDA-MB-231, control and PML- KD cells for TWIST1 & TWIST2 expression. Results are mean SD (n = 3). t-Test **P-value ≤0.05. (B) Inverse correlation of PML–TWIST2 RNA expression levels (log2 microarray from the Metabric dataset). (C) Confocal images (representative of four experiments) of co- localization between PML isoform IV, fused to mRED, and TWIST2 (upper panels) or SNAI2 (lower panels) fused to GFP (scale bar 5 lm). (D) Western blotting (WB) of co-immunoprecipitation of endogenous PML and TWIST2 proteins in MDA-MB 231 cells. Input (IN) or immuno- precipitated (IP) proteins were detected by an anti-PML (IP : PML) or IgG (IP : IgG) used as negative control (n = 2). (E) Co- immunoprecipitation of PML with TWIST1, TWIST2, SNAIL1, and SLUG (SNAI2) following co-expression in HEK293T cells. Input (IN) or anti- PML antibody immunoprecipitated (IP) proteins were detected by anti-GFP (WB : GFP; n = 2).

    Journal: Molecular oncology

    Article Title: Promyelocytic leukemia protein regulates angiogenesis and epithelial-mesenchymal transition to limit metastasis in MDA-MB-231 breast cancer cells.

    doi: 10.1002/1878-0261.13501

    Figure Lengend Snippet: Fig. 3. PML interacts with EMT factors and negatively correlates with TWIST2 expression. (A) qRT-PCR of MDA-MB-231, control and PML- KD cells for TWIST1 & TWIST2 expression. Results are mean SD (n = 3). t-Test **P-value ≤0.05. (B) Inverse correlation of PML–TWIST2 RNA expression levels (log2 microarray from the Metabric dataset). (C) Confocal images (representative of four experiments) of co- localization between PML isoform IV, fused to mRED, and TWIST2 (upper panels) or SNAI2 (lower panels) fused to GFP (scale bar 5 lm). (D) Western blotting (WB) of co-immunoprecipitation of endogenous PML and TWIST2 proteins in MDA-MB 231 cells. Input (IN) or immuno- precipitated (IP) proteins were detected by an anti-PML (IP : PML) or IgG (IP : IgG) used as negative control (n = 2). (E) Co- immunoprecipitation of PML with TWIST1, TWIST2, SNAIL1, and SLUG (SNAI2) following co-expression in HEK293T cells. Input (IN) or anti- PML antibody immunoprecipitated (IP) proteins were detected by anti-GFP (WB : GFP; n = 2).

    Article Snippet: TWIST1, TWIST2, SNAIL1 (Addgene #16225 [16]), and SLUG (Addgene #25696) were fused to GFP-C (Clontech). mCherry LEGO-C2 was provided by K. Weber.

    Techniques: Expressing, Quantitative RT-PCR, Control, RNA Expression, Microarray, Western Blot, Immunoprecipitation, Negative Control

    Fig. 3. PML interacts with EMT factors and negatively correlates with TWIST2 expression. (A) qRT-PCR of MDA-MB-231, control and PML- KD cells for TWIST1 & TWIST2 expression. Results are mean SD (n = 3). t-Test **P-value ≤0.05. (B) Inverse correlation of PML–TWIST2 RNA expression levels (log2 microarray from the Metabric dataset). (C) Confocal images (representative of four experiments) of co- localization between PML isoform IV, fused to mRED, and TWIST2 (upper panels) or SNAI2 (lower panels) fused to GFP (scale bar 5 lm). (D) Western blotting (WB) of co-immunoprecipitation of endogenous PML and TWIST2 proteins in MDA-MB 231 cells. Input (IN) or immuno- precipitated (IP) proteins were detected by an anti-PML (IP : PML) or IgG (IP : IgG) used as negative control (n = 2). (E) Co- immunoprecipitation of PML with TWIST1, TWIST2, SNAIL1, and SLUG (SNAI2) following co-expression in HEK293T cells. Input (IN) or anti- PML antibody immunoprecipitated (IP) proteins were detected by anti-GFP (WB : GFP; n = 2).

    Journal: Molecular oncology

    Article Title: Promyelocytic leukemia protein regulates angiogenesis and epithelial-mesenchymal transition to limit metastasis in MDA-MB-231 breast cancer cells.

    doi: 10.1002/1878-0261.13501

    Figure Lengend Snippet: Fig. 3. PML interacts with EMT factors and negatively correlates with TWIST2 expression. (A) qRT-PCR of MDA-MB-231, control and PML- KD cells for TWIST1 & TWIST2 expression. Results are mean SD (n = 3). t-Test **P-value ≤0.05. (B) Inverse correlation of PML–TWIST2 RNA expression levels (log2 microarray from the Metabric dataset). (C) Confocal images (representative of four experiments) of co- localization between PML isoform IV, fused to mRED, and TWIST2 (upper panels) or SNAI2 (lower panels) fused to GFP (scale bar 5 lm). (D) Western blotting (WB) of co-immunoprecipitation of endogenous PML and TWIST2 proteins in MDA-MB 231 cells. Input (IN) or immuno- precipitated (IP) proteins were detected by an anti-PML (IP : PML) or IgG (IP : IgG) used as negative control (n = 2). (E) Co- immunoprecipitation of PML with TWIST1, TWIST2, SNAIL1, and SLUG (SNAI2) following co-expression in HEK293T cells. Input (IN) or anti- PML antibody immunoprecipitated (IP) proteins were detected by anti-GFP (WB : GFP; n = 2).

    Article Snippet: TWIST1, TWIST2, SNAIL1 (Addgene #16225 [16]), and SLUG (Addgene #25696) were fused to GFP-C (Clontech). mCherry LEGO-C2 was provided by K. Weber.

    Techniques: Expressing, Quantitative RT-PCR, Control, RNA Expression, Microarray, Western Blot, Immunoprecipitation, Negative Control